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Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
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Pooled shRNA screenings: experimental approach.

Ruth Rodriguez-Barrueco1, Netonia Marshall, Jose M Silva

  • 1Institute for Cancer Genetics, Columbia University, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 30, 2013
PubMed
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RNA interference (RNAi) enables genome-wide loss-of-function studies. Pooled RNAi screens using efficient shRNA plasmids allow comprehensive genetic analysis to identify genes involved in biological processes.

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • RNA interference (RNAi) is a key tool for functional genomics.
  • Arrayed short hairpin RNA (shRNA) libraries enable genome-wide loss-of-function studies.
  • Advancements in shRNA plasmid efficiency facilitate pooled genetic screening.

Purpose of the Study:

  • To describe the methodology for conducting pooled shRNA screens.
  • To outline a pipeline for genome-wide functional interrogation.
  • To leverage efficient shRNA technology for biological discovery.

Main Methods:

  • Development and utilization of arrayed shRNA libraries.
  • Implementation of pooled screening strategies.
  • Application of efficient shRNA plasmids for genetic screens.

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MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
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MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

Published on: December 21, 2011

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
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Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

Related Experiment Videos

Last Updated: May 14, 2026

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
14:51

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
09:12

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

Published on: December 21, 2011

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

Main Results:

  • Pooled shRNA screens allow for the investigation of the entire genome.
  • Efficient shRNA plasmids enhance the feasibility of pooled genetic screens.
  • This approach identifies genes relevant to diverse biological processes.

Conclusions:

  • Pooled shRNA screening is a powerful strategy for genome-wide functional studies.
  • Efficient shRNA technology enables comprehensive genetic screens.
  • This methodology facilitates the discovery of novel genes in biological pathways.