Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 14, 2026

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
08:28

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

Published on: September 4, 2017

Macroporous silk fibroin cryogels.

Fatih Ak1, Zeynep Oztoprak, Ilknur Karakutuk

  • 1Department of Chemistry, Istanbul Technical University, 34469 Istanbul, Turkey.

Biomacromolecules
|January 31, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quadruple Shape-Memory Organohydrogels with Adjustable Trigger Temperatures.

ACS omega·2025
Same author

Silk Fibroin-Based Multiple-Shape-Memory Organohydrogels.

ACS applied materials & interfaces·2024
Same author

Cryogelation reactions and cryogels: principles and challenges.

Turkish journal of chemistry·2024
Same author

Silk Fibroin-Based Shape-Memory Organohydrogels with Semicrystalline Microinclusions.

ACS applied bio materials·2023
Same author

4D Printing of Body Temperature-Responsive Hydrogels Based on Poly(acrylic acid) with Shape-Memory and Self-Healing Abilities.

ACS applied bio materials·2023
Same author

Re-Entrant Conformation Transition in Hydrogels.

Gels (Basel, Switzerland)·2021
Same journal

Toward Sustainable Optics: Fully Biomass-Based Vitrimer Film for Near-Infrared Transparency.

Biomacromolecules·2026
Same journal

Poly(furfuryl alcohol) as a Surface Modifier for Cellulose Nanocrystals Reinforced HDPE Nanocomposites.

Biomacromolecules·2026
Same journal

Cutinase Adsorption to Polyester Surfaces Modulates Dissolved Oligomer Speciation during Enzymatic Depolymerization.

Biomacromolecules·2026
Same journal

Controlled Synthesis of Sulfated Glycopolypeptides as Heparin Mimics via <i>N</i>-Carboxyanhydride ROP for Anticoagulant Property.

Biomacromolecules·2026
Same journal

Ligno-Suberized Fruit Periderm as a Mechanically Reinforced Biomacromolecular Composite.

Biomacromolecules·2026
Same journal

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
See all related articles

Silk fibroin cryogels were created using ethylene glycol diglycidyl ether (EGDE) at subzero temperatures. These elastic cryogels form porous scaffolds with tunable mechanical properties, suitable for bone tissue engineering.

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Materials Engineering

Background:

  • Silk fibroin is a natural polymer with potential for biomedical applications.
  • Developing advanced materials with tunable properties is crucial for tissue engineering.
  • Cryogelation is a promising technique for creating porous biomaterials.

Purpose of the Study:

  • To synthesize silk fibroin cryogels with tunable properties.
  • To investigate the effect of synthesis parameters on cryogel structure and mechanical strength.
  • To evaluate the potential of these cryogels as bone scaffold materials.

Main Methods:

  • Silk fibroin solutions (4.2-12.6%) were cryogelated at subzero temperatures (-5 to -22 °C) with ethylene glycol diglycidyl ether (EGDE).
  • Conformational changes of fibroin were induced by EGDE, leading to gelation.

More Related Videos

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
06:11

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

Published on: February 26, 2019

Related Experiment Videos

Last Updated: May 14, 2026

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
08:28

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

Published on: September 4, 2017

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
06:11

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

Published on: February 26, 2019

  • Freeze-drying was used to obtain porous scaffolds from the cryogels.
  • Mechanical properties (compressive strength, modulus) and pore structure were analyzed.

    • Main Results:

    • Elastic silk fibroin cryogels were successfully synthesized.
    • EGDE induced fibroin's transition to a β-sheet structure, facilitating gelation.
    • Freeze-dried scaffolds exhibited interconnected pores (10-50 μm), tunable by synthesis parameters.
    • Mechanical properties improved with decreasing pore diameter, increasing with higher fibroin/EGDE concentrations.
    • Scaffolds from 12.6% fibroin showed a high compressive modulus (50 MPa).

    Conclusions:

    • Silk fibroin cryogels offer tunable elasticity and mechanical strength.
    • Controlled pore structures can be achieved through cryogelation and freeze-drying.
    • High-performance silk fibroin scaffolds demonstrate significant potential for bone regeneration applications.