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Abnormal hematopoietic phenotypes in Pim kinase triple knockout mice
Ningfei An1, Andrew S Kraft, Yubin Kang
1Division of Hematology-Oncology, Department of Medicine, Medical University of South Carolina, 86 Jonathan Lucas Street, Hollings Cancer Center Rm# HO307, Charleston, SC 29425, USA.
Journal of Hematology & Oncology
|January 31, 2013
Summary
Pim kinases are crucial for hematopoietic stem cell (HSC) function, impacting their expansion, self-renewal, and long-term repopulation capabilities. Deletion of Pim kinases impairs HSCs, affecting blood cell development.
Area of Science:
- Hematology
- Molecular Biology
- Oncology
Background:
- Pim kinases (Pim1, Pim2, Pim3) are serine/threonine oncogenic kinases.
- They regulate B- and T-cell responses to growth factors.
- Their role in hematopoietic stem cells (HSCs) is largely unknown.
Purpose of the Study:
- Investigate the role of Pim kinases in hematopoiesis.
- Determine the function of Pim kinases in HSCs.
Main Methods:
- Used Pim1-/-2-/-3-/- triple knockout (TKO) mice.
- Assessed peripheral blood parameters and HSC function via transplantation.
- Evaluated HSC proliferation and apoptosis using BrdU, Ki67, and caspase 3 assays.
Main Results:
- Pim TKO mice showed reduced platelet counts and microcytosis.
- HSCs from Pim TKO mice had impaired colony-forming ability and long-term repopulation capacity.
- Reduced HSC proliferation and increased apoptosis were observed in Pim TKO mice.
Conclusions:
- Pim kinases are essential for hematopoietic lineage development.
- Pim kinases play a critical role in HSC expansion, self-renewal, and long-term repopulation.
