Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Health professions education in a constrained financing era: implications of the One Big Beautiful Bill Act.

Academic medicine : journal of the Association of American Medical Colleges·2026
Same author

Genotoxicity profiling reveals distinct platform-and cell type-specific effects in therapeutic gene editing for genetic hyperinflammation.

Cell stem cell·2026
Same author

MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryos.

Nature communications·2026
Same author

Click-to-Release Reactions for Tertiary Amines and Pyridines.

Journal of the American Chemical Society·2026
Same author

Aryl azopyrroles as visible light photoswitchable TRPA1 ligands.

Chemical science·2025
Same author

Combined Inductive and Dispersion Effects Enhance Bioorthogonal Reactivity of Tetrazines Toward Isonitriles.

Angewandte Chemie (International ed. in English)·2025
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
See all related articles

Related Experiment Video

Updated: May 14, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

Efficient genome editing in zebrafish using a CRISPR-Cas system.

Woong Y Hwang1, Yanfang Fu, Deepak Reyon

  • 1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

Nature Biotechnology
|January 31, 2013
PubMed
Summary
This summary is machine-generated.

The CRISPR-Cas system enables targeted genetic modification in zebrafish embryos. This bacterial defense mechanism achieves high efficiency for in vivo gene editing applications.

More Related Videos

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Related Experiment Videos

Last Updated: May 14, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems are bacterial defense mechanisms against foreign nucleic acids.
  • Bacterial type II CRISPR systems, specifically the Cas9 endonuclease, have been engineered with guide RNAs for targeted DNA cleavage in cellular systems.

Purpose of the Study:

  • To investigate the efficacy of the CRISPR-Cas system for inducing targeted genetic modifications in vivo.
  • To compare the efficiency of CRISPR-Cas gene editing in zebrafish embryos with existing technologies like zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs).

Main Methods:

  • Adaptation of bacterial type II CRISPR-Cas system components (Cas9 endonuclease and guide RNA) for use in eukaryotic systems.
  • Introduction of the CRISPR-Cas system into zebrafish embryos to achieve site-specific DNA cleavage and subsequent genetic modification.

Main Results:

  • Demonstration that the CRISPR-Cas system functions effectively in vivo within zebrafish embryos.
  • Achieved targeted genetic modifications with efficiencies comparable to those obtained using established gene editing tools such as ZFNs and TALENs.

Conclusions:

  • The CRISPR-Cas system is a potent tool for in vivo genome engineering in zebrafish embryos.
  • CRISPR-Cas technology offers a highly efficient and versatile alternative for genetic manipulation in developmental biology research.