An optimal polymerization conditions for poly-human placenta hemoglobin with lower mean molecular weight
Wentao Zhou1, Lanzhen Zhao, Jinfeng Wang
1School of Pharmaceutical Science & Technology, Tianjin University , Tianjin , P. R. China.
Objectives:
To reduce the mean molecular weight [Formula: see text] and to increase the effective polymerization ratio (REff) of polymerized human placenta hemoglobin (PolyPHb).
Methods:
Three factors of GDA-PolyPHb process such as the approach of feeding GDA (FGDA), hemoglobin concentration ([Hb]) and the molar ratio of GDA, and hemoglobin(RGDA:Hb) were investigated. Finally, the expansion experiments were conducted with optimal conditions.
Results:
The data showed that the HBOCs with the REff of 67.35% and lower [Formula: see text] of 162.70 kDa were prepared by optimal conditions.
Conclusion:
Compared to original process, the optimal process greatly decreased the [Formula: see text] and increased the REff.
