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Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

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Published on: September 27, 2024

Versatile broadband electrode assembly for cell electroporation.

Yu-Hsuan Wu1, Delia Arnaud-Cormos, Maura Casciola

  • 1Mork Family Department of Chemical Engineering and Materials Science, VsOE, USC, Los Angeles, CA, USA.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|February 1, 2013
PubMed
Summary

A novel electrode assembly for cell electroporation was developed and validated using pulsed electric fields. This versatile system demonstrates broadband behavior, enhancing cellular delivery efficiency.

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Area of Science:

  • Biomedical Engineering
  • Electrophysiology

Background:

  • Cell electroporation is a key technique for genetic material delivery.
  • Existing electrode assemblies may have limitations in versatility and performance.

Purpose of the Study:

  • To propose and validate a versatile electrode assembly for cell electroporation.
  • To demonstrate the broadband characteristics of the proposed delivery system.

Main Methods:

  • Development of a novel electrode assembly for cell electroporation.
  • Conducting biological cell electroporation experiments.
  • Utilizing 2.5 ns and 5 ns, 10 MV/m pulsed electric fields.
  • Performing electromagnetic, time domain, and frequency analyses.

Main Results:

  • The proposed electrode assembly proved versatile for cell electroporation.
  • Experiments validated the delivery system's efficacy.
  • Electromagnetic, time domain, and frequency analyses confirmed broadband behavior.

Conclusions:

  • The developed electrode assembly is a versatile and effective tool for cell electroporation.
  • The system's broadband characteristics are confirmed, suggesting broad applicability.