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Updated: May 14, 2026

Identification of protein complexes with quantitative proteomics in S. cerevisiae
Published on: March 4, 2009
Molecular characterization of Saccharomyces cerevisiae α-pheromone by mass spectrometry-based peptidomics
Eda Bener Aksam1, Martijn W H Pinkse, Peter D E M Verhaert
1Department of Biotechnology, Kluyver Laboratory & Netherlands Proteomics Centre, Delft University of Technology, Delft, The Netherlands.
Abstract:
Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In addition to the mature intact α-pheromones, also post-translationally modified α-pheromone peptides and fragments thereof are found to be present in the culture medium. This molecular analytical technique is complementary to the recently described quantitation method by Rogers et al. (2012, FEMS Yeast Res. 12:668) based on ELISA.
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