Related Experiment Video
Updated: May 14, 2026
Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury
Published on: October 6, 2020
Peroneal nerve reconstruction by using glycerol-preserved veins: histological and functional assessment in rats
Armando dos Santos Cunha1, Marcio Paulino Costa, Ciro Ferreira da Silva
1Posgraduate Program in Medicine, Surgical Clinic, USP, Sao Paulo, Brazil. armandocunha@hotmail.com
Purpose:
To compare the degree of neural regeneration in rats upon interposition of autologous nerve graft, autogenous vein, glycerol-preserved autogenous vein, and glycerol-preserved allogeneic vein using qualitative and quantitative histological analyses as well as functional assessments.
Methods:
Peroneal nerves were reconstructed differently in four groups of animals. Functional assessments were performed pre- and postoperatively for a period of six weeks. After six weeks, the animals were sacrificed and histological evaluations were performed.
Results:
Histological patterns of autogenous veins without preservation showed pronounced neoangiogenesis and extensive axonal rarefaction, as confirmed by axonal counting and functional assessments. Glycerol-preserved veins had results similar to the control.
Conclusions:
Glycerol-preserved autogenous or allogeneic veins showed similar results to autograft results. The autogenous vein (without preservation in glycerol) presented histological and functional outcomes statistically lower than other groups.
