Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 14, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Tissue microarray design and construction for scientific, industrial and diagnostic use.

Daniela Pilla1, Francesca M Bosisio, Roberto Marotta

  • 1Department of Pathology, San Gerardo Hospital, Via Pergolesi 33, 20900, Monza, Italy.

Journal of Pathology Informatics
|February 2, 2013
PubMed
Summary

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics.

PloS one·2026
Same author

ER-associated degradation pathway protein SEL1L plays an evolutionarily conserved role in platelet adhesion.

The Journal of clinical investigation·2026
Same author

Milk and honey: how skin changes reveal the deeper dangers of nutritional deficiencies.

Acta clinica Belgica·2026
Same author

Prevention of Transfusion-Transmitted Malaria and Chagas Disease in Non-Endemic Countries: An 8-Year Study of Seroprevalence Among Donors at Risk in Tuscany (Central Italy).

Pathogens (Basel, Switzerland)·2026
Same author

Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality.

Nature communications·2025
Same author

Beyond Tissue in the Era of Liquid Biopsy: Pathologists' Perspectives and Insights.

Cytopathology : official journal of the British Society for Clinical Cytology·2025

Tissue Micro Array (TMA) technology is validated for high-throughput research, diagnostics, and industry. Addressing potential errors ensures accurate specimen identification and data pairing for reliable results.

Area of Science:

  • Histopathology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Tissue Micro Array (TMA) technology, while widely used, has lacked rigorous scientific scrutiny regarding error sources.
  • Specimen identification and data pairing accuracy in TMA workflows remain insufficiently reported and validated.

Purpose of the Study:

  • To establish recognized quality standards for TMA technology in research, diagnostics, and industrial applications.
  • To systematically identify and mitigate sources of error in TMA processes.

Main Methods:

  • Implemented a barcode-driven data input system throughout the entire TMA workflow.
  • Developed a method for matching TMA design with virtual images and segmenting images back to individual cases and data.
  • Provided mathematical validation for the correspondence between TMA design, whole slide images, and patient data.
Keywords:
Diagnosticpathologytissue micro arraytissue microarrayvirtual slidewhole slide image

More Related Videos

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Related Experiment Videos

Last Updated: May 14, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Main Results:

  • Systematic error identification and control were achieved using barcode-driven data input.
  • Accurate segmentation and data pairing were demonstrated, linking images to individual cases and associated data.
  • Mathematical principles were applied to validate sample-data correspondence on a per-sample basis.

Conclusions:

  • High-throughput TMA technology is a safe and efficient method when all error sources are identified and addressed.
  • The validated methods enhance the reliability of TMA for research, diagnostics, and industrial purposes.