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[Standardizing a central nervous system tumor DNA biobank]
Diego Méndez-Rosito1, Edgar Rangel-López, María Lucinda Aguirre-Cruz
1Departamento de Neurocirugía, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, La Fama, Tlalpan, México, D.F., Mexico. diegomendezrosito@gmail.com
Background:
Brain tumors are one of the leading cancers worldwide; in the National Institute of Neurology and Neurosurgery (INNN) these tumors are the leading cause of morbitity and mortality.
Objective:
Standardize biopsies, colletion, processing and storage biologic material of molecular studies.
Methods:
with a previously signed surgical consent, a tumor and blood biopsy was done to 134 patients. Their DNA was extracted and a database was filled considering technical, ethical and legal aspects. In order to have optimal biologic material the procedure was standardized between the surgical and research laboratory teams.
Results:
The biopsy, transportation, processing and storage were standardized. 134 patients were included (67 male and 67 female) with an average age of 46.28 years (range 15-81). The most frequently biopsied tumor was the meningioma (42%). The integrity of the obtained material was determined by agarose gel electrophoretic analysis.
Conclusion:
the INNN biobank has a standardized system that biopsies, processes and stores optimum quality biologic material that will be the basis of future molecular studies.
