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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Overview of Exosomes01:36

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

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Related Experiment Video

Updated: May 14, 2026

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Methodologies to decipher the cell secretome.

Paromita Mukherjee1, Sridhar Mani

  • 1Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, 10461, USA.

Biochimica Et Biophysica Acta
|February 5, 2013
PubMed
Summary

This review explores methods for identifying the cell secretome, which includes proteins secreted by cells. Understanding these secretory proteins is crucial for cell communication and migration.

Keywords:
SecretomeSecretory proteinSignal peptides

Related Experiment Videos

Last Updated: May 14, 2026

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • The cell secretome comprises transmembrane and secreted proteins, constituting ~13-20% of the human proteome.
  • Secretory proteins are vital for intercellular communication, cell signaling, and migration.

Purpose of the Study:

  • To comprehensively review and discuss methodologies for identifying cell secretomes.
  • To analyze the advantages and disadvantages of various secretome identification techniques.

Main Methods:

  • Serial Analysis of Gene Expression (SAGE)
  • DNA microarrays
  • Antibody arrays and bead-based arrays
  • Mass spectrometry
  • RNA sequencing
  • Secretion traps (yeast, bacterial, mammalian)

Main Results:

  • A wide array of techniques exists for secretome analysis, each with unique strengths and limitations.
  • The choice of method depends on specific research objectives and experimental constraints.

Conclusions:

  • Accurate secretome identification is essential for understanding cellular functions.
  • Selecting the appropriate methodology is critical for successful secretome research.