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Updated: May 14, 2026
Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
Published on: December 23, 2022
Analysis of interactions between proteins and fatty acids or cholesterol using a fatty acid/cholesterol pull-down
E Beck-García1, K Beck-García, A Schlosser
1Department of Molecular Immunology, Institute for Biology III, BIOSS Center for Biological Signalling Studies, Centre for Chronic Immunodeficiency (CCI), Albert Ludwigs University Freiburg and Max Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
Abstract:
Specific interactions between the polar head groups of membrane lipids and proteins have been described previously. In contrast, the specificity of the interaction between lipid acyl chains with proteins is less understood. By combining a fatty acid or cholesterol pull-down assay with Western blot analysis or mass spectrometry, we identified transmembrane and cytosolic proteins that bound preferentially to short or long acyl chains or to cholesterol. Thus, this approach allows identification of specific fatty acid-protein or cholesterol-protein interactions.
