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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Gene Conversion02:08

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Updated: May 14, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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Published on: December 29, 2021

Computer programs and methodologies for the simulation of DNA sequence data with recombination.

Miguel Arenas1

  • 1Centre for Molecular Biology "Severo Ochoa," Consejo Superior de Investigaciones Científicas Madrid, Spain.

Frontiers in Genetics
|February 5, 2013
PubMed
Summary
This summary is machine-generated.

This review guides researchers in selecting computer simulators for DNA sequence evolution with recombination. It helps ensure biologically meaningful simulations and highlights the impact of ignoring recombination in evolutionary analyses.

Keywords:
DNA sequencesrecombinationrecombination breakpointsrecombination hotspotsrecombination phylogenetic biassimulation

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Recombineering Homologous Recombination Constructs in Drosophila
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Published on: July 13, 2013

Area of Science:

  • Evolutionary Biology
  • Computational Biology
  • Genomics

Background:

  • Computer simulations are vital for hypothesis testing and parameter estimation in evolutionary biology.
  • Simulating DNA sequences with recombination is crucial for understanding genome structure and evolutionary processes.
  • Numerous computer simulators exist, but choosing the right one for biologically meaningful recombination simulations is challenging.

Purpose of the Study:

  • To provide a practical guide to commonly used computer programs and methodologies for simulating DNA sequences with recombination.
  • To aid researchers in designing effective computer simulation experiments.
  • To review studies on the impact of ignoring recombination in evolutionary analyses.

Main Methods:

  • Review of existing computer programs for DNA sequence simulation with recombination.
  • Analysis of methodologies for simulating both coding and non-coding DNA sequences.
  • Compilation of studies investigating the consequences of neglecting recombination.

Main Results:

  • Identified key computer programs and methodologies for simulating DNA sequences with recombination.
  • Highlighted the critical importance of selecting appropriate simulation tools.
  • Summarized the impact of ignoring recombination on phylogenetic and ancestral sequence reconstruction.

Conclusions:

  • Proper selection of recombination simulation tools is essential for biologically relevant research.
  • Ignoring recombination can lead to inaccurate evolutionary analyses.
  • Alternative analytical methods accounting for recombination are available and reviewed.