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Updated: May 14, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Simultaneous profiling of polar lipids by supercritical fluid chromatography/tandem mass spectrometry with
Jae Won Lee1, Shin Nishiumi, Masaru Yoshida
1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Abstract:
Supercritical fluid chromatography/tandem mass spectrometry (SFC/MS/MS) with methylation was used for the simultaneous profiling of diverse polar lipids in a mixture. A high throughput, high resolution analysis of nineteen classes of polar lipids including phospholipids, lysophospholipids, and sphingolipids was performed in 6 min. Methylation by trimethylsilyl-diazomethane suppressed peak tailing and improved detection sensitivity of phosphatidylserine (PS), phosphatidic acid (PA), lysophosphatidylserine (LPS), lysophosphatidylinositol (LPI), lysophosphatidic acid (LPA), ceramide-1-phosphate (Cer1P), sphingosine-1-phosphate (So1P), and sphinganine-1-phosphate (Sa1P). The limits of detection for PS, PA, LPS, LPI, LPA, Cer1P, So1P, and Sa1P were enhanced 7.5-, 26.7-, 600-, 116.7-, 500-, 75-, 3000-, and 4500-fold, respectively. Global qualitative and quantitative analysis of not only the high-abundance species but also the low-abundance species in the polar lipids was achieved. When the method was applied to mouse liver, 4 PSs, 24 PAs, 3 lysophosphatidylethanolamines, 11 LPSs, 6 lysophosphatidylglycerols, 4 LPIs, 13 LPAs, 7 sphingomyelins, 11 Cer1Ps, So1P, and Sa1P were additionally analyzed. Furthermore, the quantification of various molecular species in each polar lipid was carried out.
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