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The origin of circulating CD36 in type 2 diabetes
M J Alkhatatbeh1, A K Enjeti, S Acharya
11] Cancer Research Unit, School of Biomedical Sciences and Pharmacy, Faculty of Health, the University of Newcastle, Newcastle, NSW, Australia [2] Hunter Medical Research Institute, New Lambton, NSW, Australia.
Nutrition & Diabetes
|February 6, 2013
Summary
Elevated CD36 microparticles (MPs) are a novel biomarker for type 2 diabetes mellitus (T2DM). In T2DM, these MPs originate mainly from erythrocytes, unlike in controls where they stem from endothelial cells.
Area of Science:
- Biochemistry
- Cell Biology
- Diabetes Research
Background:
- Elevated CD36, a fatty acid transporter, is a potential biomarker for type 2 diabetes mellitus (T2DM).
- Circulating CD36 is associated with cellular microparticles (MPs).
Purpose of the Study:
- Determine absolute levels of CD36+ MPs in T2DM patients.
- Identify the cellular origins of CD36+ MPs in T2DM.
Main Methods:
- Ex vivo case-control study of T2DM patients and matched controls.
- Flow cytometry to analyze MP surface markers (CD36, CD41, CD235a, CD14, CD105, phosphatidyl serine).
- Enzyme-linked immunosorbent assay to quantify absolute CD36 protein concentrations.
Main Results:
- CD36+ MP levels were significantly higher in T2DM patients (P<0.00001).
- In T2DM, CD36+ MPs were primarily from erythrocytes (CD235a+); in controls, from endothelial cells (CD105+).
- Plasma CD36+ MP levels were a better diabetes biomarker than CD36 protein concentration (P=0.009 vs P=0.398).
Conclusions:
- CD36+ MP levels and cellular origins differ between T2DM patients and controls.
- These MPs may represent distinct biological vectors contributing to T2DM pathology.
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