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Published on: May 1, 2019
Nonradioactive in vitro assays for histone deacetylases
Alexander-Thomas Hauser1, Julia M Gajer Née Wagner, Manfred Jung
1Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|February 6, 2013
Summary
This chapter details non-radioactive in vitro assays for detecting histone deacetylase (HDAC) activity, suitable for high-throughput screening and monitoring in cancer cells and blood. These methods aid in developing HDAC inhibitors for disease treatment.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Histone deacetylases (HDACs) regulate gene expression, making them key targets for treating cancers and other diseases.
- Understanding HDAC activity is crucial for developing targeted therapies.
Purpose of the Study:
- To present non-radioactive in vitro assays for detecting HDAC activity.
- To adapt these assays for high-throughput screening (HTS) in microtiter plates.
- To provide protocols for monitoring HDAC activity in cancer cells and peripheral white blood cells.
Main Methods:
- Development and description of in vitro assays for HDAC activity.
- Adaptation of assays for microtiter plate-based, high-throughput screening.
- Protocols for monitoring HDAC activity in biological samples (cancer cells, white blood cells).
Main Results:
- Established non-radioactive assays for reliable HDAC activity detection.
- Demonstrated suitability of assays for high-throughput screening.
- Provided practical protocols for cellular and ex vivo HDAC activity monitoring.
Conclusions:
- The presented in vitro assays offer robust, non-radioactive methods for HDAC activity assessment.
- These assays are valuable tools for drug discovery and disease research targeting HDACs.
- The protocols facilitate reliable and reproducible results in various biological contexts.
