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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 14, 2026

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
07:29

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry

Published on: October 6, 2023

Single cell microRNA analysis using microfluidic flow cytometry.

Meiye Wu1, Matthew Piccini, Chung-Yan Koh

  • 1Department of Biotechnology and Bioengineering, Sandia National Laboratory, Livermore, CA, USA.

Plos One
|February 6, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic flow-FISH method for detecting microRNAs (miRNAs) in single cells. This technique enables simultaneous analysis of miRNAs and their target gene products, advancing single-cell miRNA research.

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Last Updated: May 14, 2026

Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
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Published on: June 23, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression with cell-specific functions.
  • Studying miRNA expression and localization at single-cell resolution is essential for understanding cellular processes.
  • Existing methods often lack the ability to analyze miRNAs alongside their targets in the same cell.

Purpose of the Study:

  • To develop a novel microfluidic approach for single-cell miRNA detection and analysis.
  • To enable simultaneous measurement of miRNA and its potential target gene products within individual cells.
  • To establish a rapid and reagent-efficient method for miRNA research.

Main Methods:

  • Development of a microfluidic flow-FISH (flow fluorescent in situ hybridization) technique.
  • Integration of locked-nucleic acid probes for sensitive miRNA detection.
  • Combination with rolling circle amplification for enhanced signal amplification.
  • Application of flow cytometry for single-cell analysis of miRNA and gene products.

Main Results:

  • Successful simultaneous detection and localization of microRNA (miR155) and CD69 in stimulated Jurkat cells.
  • Demonstration of the method's applicability in a cellular model.
  • The flow-FISH method is completed in approximately 10 hours.
  • The technique requires minimal reagent volumes (170 nL per condition).

Conclusions:

  • The novel microfluidic flow-FISH method provides direct detection of miRNA in single cells via flow cytometry.
  • This approach allows for the simultaneous analysis of miRNA and gene products in the same cell.
  • The method is rapid, requires minimal reagents, and offers a powerful tool for single-cell miRNA research.