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Updated: May 14, 2026
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 10, 2009
Highly efficient virus-induced gene silencing in apple and soybean by apple latent spherical virus vector and
Noriko Yamagishi1, Nobuyuki Yoshikawa
1Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka, Japan.
Abstract:
Virus-induced gene silencing (VIGS) is an effective tool for the analysis of the gene function in plants within a short time. However, in woody fruit tree like apple, some of Solanum crops, and soybean, it is generally difficult to inoculate virus vector by conventional inoculation methods. Here, we show efficient VIGS in apple and soybean by Apple latent spherical virus (ALSV) vector and biolistic inoculation. The plants inoculated with ALSV vectors by particle bombardment showed uniform silenced phenotypes of target genes within 2-3 weeks post inoculation.
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