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Updated: May 14, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Separation of chitooligosaccharides in acidic solution by capillary electrophoresis
Toshiaki Hattori1, Nobuhiro Anraku, Ryo Kato
1Department of Electric and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi, Japan. thattori@ee.tut.ac.jp
Abstract:
Capillary electrophoresis (CE) of chitooligosaccharides (COS) in aqueous solution is effective for their separation from other saccharides. However, COS easily adsorb on negatively charged surfaces, such as fused silica capillaries in acidic solutions. Conventional photometric detection cannot be applied directly because saccharides do not absorb ultraviolet or visible light. Here, we describe a simple CE of COS in an acidic solution using a positively charged capillary coated with N-trimethoxypropyl-N,N,N-trimethylammonium chloride and indirect photometric detection with crystal violet nitrate background solution.
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