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[Expression changes of MEK in lung of rats subjected to brain ischemia]
Sheng-Lan Wang1, Qi-Qin Dan, Rong Rong
1Department of Respiration, Affiliated Kunhua Hospital of Kunming Science and Technology University, Kunming 650032, China.
Objective:
To explore the expression changes of mitogen extracellular kinase (MEK) in injured lung after brain ischemia in rats.
Methods:
Adult SD rats were assigned randomly to sham operation group and brain ischemia lung injury (BILI) group. Rats in BILI group were subjected brain ischemia and allowed to survived 3 d. Pathalogical changes in lung were indicated by HE staining. The MEK expression was determined by RT-PCR and Western blot technique.
Results:
After brain ischemia, the bulk of inflammatory cells invaded into lung were observed. Upregulated level of MEK mRNA and protein were found at 3 days after ischemia (P<0.05).
Conclusion:
The upregulated expression of MEK implied that the MEK may play some roles in lung injury after brain ischemia.
