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Updated: May 14, 2026
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
Long non-coding RNA targeting and transcriptional de-repression.
Marc S Weinberg1, Kevin V Morris
1Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.
Nucleic Acid Therapeutics
|February 9, 2013
Summary
Long non-coding RNAs (lncRNAs) regulate gene expression by recruiting epigenetic silencing complexes. Inhibiting these lncRNAs can activate gene expression, revealing a new RNA-based epigenetic network.
Area of Science:
- Molecular Biology
- Epigenetics
- Genomics
Background:
- The human genome is largely transcribed, with many non-coding RNAs playing regulatory roles.
- Non-coding RNAs, particularly long non-coding RNAs (lncRNAs), are emerging as key modulators of protein-coding gene function.
- This regulation extends beyond imprinted genes to various differentiated cells.
Purpose of the Study:
- To investigate the role of lncRNAs in epigenetic gene regulation.
- To explore the mechanism by which lncRNAs control gene transcription.
- To understand the implications of RNA-based epigenetic control.
Main Methods:
- Analysis of genomic transcription data.
- Investigating the recruitment of epigenetic silencing complexes by lncRNAs.
- Utilizing small RNA-based inhibitors, such as RNA interference, to target lncRNAs.
Main Results:
- lncRNAs can target epigenetic silencing complexes to specific genomic loci.
- Inhibition of repressor lncRNAs leads to de-repression and activation of gene expression.
- Evidence supports a widespread RNA-based epigenetic regulatory network.
Conclusions:
- lncRNAs are functional regulators of gene transcription through epigenetic mechanisms.
- Targeting lncRNAs offers a novel approach for modulating gene expression.
- This field has significant implications for cellular evolution and pharmacology.
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