[The effect of ambazone and ambazone derivatives on immunologic processes in human lymphocytes]

The influence of 1,4-benzoquinone-guanylhydrazone-thio-semicarbazone (1; ambazone), toluquinone-guanylhydrazone-thiosemicarbazone (2; ambazone 82/80) and p-(thio-semicarbacido)-diaminomethylenehydrazino benzene (3; dihydroambazone) on phytomitogen induced stimulation processes of human lymphocytes was studied. Con A- or PHA-stimulated lymphocytes of healthy probands were treated in vitro with the drugs and the DNA synthesis rate was evaluated by the use of the 3H-labelled thymidine incorporation. A concentration dependent inhibition of the DNA synthesis rate in a comparable quantity for all the tested drugs was found. DNA synthesis was inhibited nearly completely by drug application in a concentration range of 10(-4) mol/l. To check for a possible interference of mitogen stimulation and drug action, 3 was added to the lymphocyte cultures either together with PHA, prior to or after addition of the mitogen. Experiments showed, the sooner the drug acted on the stimulation process, the stronger the DNA synthesis was inhibited.