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[Development of a method for the assessment of melamine in urines by GC-MS/MS. An attempt to define a reference
C Romano1, E Marchetti, A Genova
1Dipartimento di Traumatologia, Ortopedia e Medicina del Lavoro, Università di Torino, Via Zuretti 29, 10126 Torino, Italy.
Abstract:
Melamine is used in the synthesis of resins used for the manufacture of laminates, plastics and coatings, including materials in contact with food such as crockery. A method for the assessment of melamine by GC-MSMS in urine samples was developed and tested in 100 non-occupationally exposed subjects: 63 females (14-66) and 37 males (15-65). The LOD and LOQ were 4 microg/l and 13.4 microg/l respectively. Results showed traces of melamine in the urines of 21% of subjects monitored (10-29.7 microg/l). Presence of melamine and time since last meal at sampling were inversely related (p = 0.021). In addition a significant correlation (p = 0.00) between the presence of the substance in urines and the use of melamine dishes was shown.
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