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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Interactome mapping: using protein microarray technology to reconstruct diverse protein networks.

Ijeoma Uzoma1, Heng Zhu

  • 1Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Genomics, Proteomics & Bioinformatics
|February 12, 2013
PubMed
Summary
This summary is machine-generated.

Protein microarrays are versatile tools for mapping cellular interactions in systems biology. This technology aids in reconstructing complex biological networks, including protein-DNA interactions and signaling cascades, advancing our understanding of biological systems.

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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
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Last Updated: May 14, 2026

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Area of Science:

  • Systems Biology
  • Proteomics
  • Molecular Interactions

Background:

  • Systems biology aims to understand complex biological networks by characterizing cellular component interactions.
  • Protein microarrays have emerged as a significant platform in proteomics, facilitating systems biology research over the last decade.
  • The flexibility of protein microarrays allows for diverse applications in studying biochemical properties of proteins.

Purpose of the Study:

  • To review studies utilizing protein microarrays for reconstructing biological networks.
  • To highlight the application of protein microarrays in understanding diverse molecular interactions and cellular states.
  • To discuss emerging applications and future prospects of protein microarray technology.

Main Methods:

  • Analysis of numerous studies employing protein microarrays.
  • Focus on reconstruction of biological networks including protein-DNA interactions, posttranslational protein modifications (PTMs), lectin-glycan recognition, pathogen-host interactions, and signaling cascades.
  • Integration of interaction data from various molecular classes and cellular states.

Main Results:

  • Protein microarrays have been successfully used to map diverse biological interactions, contributing to network reconstruction.
  • The technology enables the integration of data across different molecular classes, offering insights into complex biological systems.
  • Demonstrated utility in studying protein-DNA interactions, PTMs, glycan recognition, host-pathogen interactions, and signaling pathways.

Conclusions:

  • Protein microarrays are a powerful and flexible technology for dissecting intricate biological networks.
  • The diverse applications provide a comprehensive view of cellular interactions, crucial for systems biology.
  • Continued development promises further advancements in understanding complex biological systems.