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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: May 14, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Extracting intracellular diffusive states and transition rates from single-molecule tracking data.

Fredrik Persson1, Martin Lindén, Cecilia Unoson

  • 1Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.

Nature Methods
|February 12, 2013
PubMed
Summary

We developed a new analytical tool to map protein interactions by analyzing single-molecule movements. This method objectively identifies how Hfq protein interacts with RNA and mRNA targets.

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Related Experiment Videos

Last Updated: May 14, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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10:43

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Published on: July 19, 2022

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12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

Area of Science:

  • Biophysics
  • Molecular Biology
  • Computational Biology

Background:

  • Intracellular protein diffusion is crucial for cellular function.
  • Understanding protein-protein and protein-RNA interactions is key to deciphering cellular mechanisms.
  • The Hfq protein plays a vital role in regulating gene expression by mediating RNA-RNA and RNA-protein interactions.

Purpose of the Study:

  • To develop a novel analytical tool for analyzing single-molecule trajectories.
  • To objectively map interactions of the Hfq protein.
  • To identify diffusive states and transition rates of intracellularly diffusing proteins.

Main Methods:

  • Utilized a variational Bayesian treatment of hidden Markov models.
  • Analyzed thousands of short single-molecule trajectories of intracellularly diffusing proteins.
  • Applied the developed method to create an interaction map for the Hfq protein.

Main Results:

  • Successfully identified the number of diffusive states and state transition rates.
  • Created an objective interaction map for the Hfq protein.
  • Demonstrated the utility of the analytical tool in a biological context.

Conclusions:

  • The developed variational Bayesian hidden Markov model approach is effective for analyzing single-molecule trajectory data.
  • The method provides an objective way to map protein interactions, exemplified by the Hfq protein.
  • This tool can advance the understanding of molecular mechanisms involving protein diffusion and interactions within cells.