Development of a bioluminescent bacteria sheet for the measurement of oxygen concentration

The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The relationship between the dissolved oxygen (DO) concentration in the biofilm and luminescence from the sheet was examined. As a conclusion, the alginate-gel film method was effective in fabricating a DO-monitoring thin film.