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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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Related Experiment Video

Updated: May 14, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Long noncoding RNAs regulate adipogenesis.

Lei Sun1, Loyal A Goff, Cole Trapnell

  • 1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 13, 2013
PubMed
Summary
This summary is machine-generated.

This study identifies 175 long noncoding RNAs (lncRNAs) crucial for adipogenesis, the process of fat cell development. These lncRNAs are essential for proper fat cell formation and function.

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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Last Updated: May 14, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Obesity prevalence drives research into adipocyte development mechanisms.
  • While protein-coding genes, mRNAs, and microRNAs are studied, long noncoding RNAs (lncRNAs) in adipogenesis remain underexplored.
  • Understanding lncRNA roles is vital for metabolic disease research.

Purpose of the Study:

  • To globally profile lncRNA expression during adipogenesis.
  • To identify lncRNAs specifically regulated during adipocyte differentiation.
  • To determine the functional significance of lncRNAs in adipogenesis.

Main Methods:

  • Transcriptome profiling of primary and cultured adipocytes and preadipocytes.
  • Identification of differentially expressed lncRNAs during adipogenesis.
  • Chromatin immunoprecipitation to assess transcription factor binding.
  • RNA interference (RNAi)-mediated loss-of-function screens.

Main Results:

  • Identified 175 lncRNAs regulated during adipogenesis.
  • Many identified lncRNAs are adipose-enriched and induced during differentiation.
  • Key adipogenic transcription factors (PPARγ, CEBPα) bind promoters of these lncRNAs.
  • Functional screens revealed lncRNAs essential for adipogenesis.

Conclusions:

  • Numerous lncRNAs are specifically regulated during adipogenesis.
  • These lncRNAs are functionally important for adipocyte development.
  • This work expands the understanding of noncoding RNA roles in metabolic processes.