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System for acquisition and real-time processing of multidimensional slit-scan flow cytometric data.
R D Robinson1, D M Wheeless, S J Hespelt
1Department of Pathology, University of Rochester Medical Center, New York 14642.
Cytometry
|January 1, 1990
Summary
A new hardware and software system enables high-speed, flexible sampling of cell contours. This system processes multidimensional slit-scan signals, offering adaptable solutions for biological research.
Area of Science:
- Biophysical instrumentation
- Cellular imaging technology
- Signal processing in biology
Background:
- High-speed sampling of multidimensional slit-scan signals (cell contours) traditionally necessitates custom hardware.
- Existing specialized hardware often lacks the adaptability for diverse instrument setups and experimental needs.
Purpose of the Study:
- To describe a flexible hardware and software system for sampling multiple slit-scan cell contours.
- To achieve high-speed data acquisition (up to 40 MHz) with 10-bit resolution.
Main Methods:
- Utilized commercially available CAMAC transient recorders and a PDP-11/83 computer.
- Incorporated custom hardware for signal conditioning and trigger generation.
- Developed a modular software system for adaptability to various hardware options.
Main Results:
- The system achieves sampling rates of up to 40 MHz with 10-bit resolution.
- Real-time digital processing extracts morphological features (width, height, area) from cell contours.
- Gated histograms are accumulated, and data/contours can be saved to disk.
Conclusions:
- The described system offers a flexible and high-performance solution for multidimensional slit-scan signal acquisition.
- Its modular design facilitates adaptation to evolving experimental requirements in cell biology.
- Enables advanced real-time analysis and data storage for cellular morphology studies.