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Production of Monoclonal Antibody against Human Nestin

Reza Hadavi1, Amir Hassan Zarnani, Negah Ahmadvand

  • 1Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Avicenna Journal of Medical Biotechnology
|February 15, 2013
PubMed

We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.

Keywords:
AntibodiesBlottingImmunohistochemistryMonoclonalNestinPeptidesWestern

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