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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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Related Experiment Video

Updated: May 14, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Epigenomics: sequencing the methylome.

Martin Hirst1

  • 1Department of Microbiology and Immunology, Centre for High-Throughput Biology, University of British Columbia, Vancouver, Canada. mhirst@bcgsc.ca

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2013
PubMed
Summary
This summary is machine-generated.

This study reviews DNA methylation sequencing techniques. It covers methods like affinity enrichment, chemical conversion, and enzymatic restriction for analyzing DNA methylation patterns.

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Last Updated: May 14, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology

Background:

  • DNA methylation is a key epigenetic modification.
  • Accurate surveying of DNA methylation patterns is crucial for understanding biological processes and disease.
  • Advancements in sequencing technologies have enabled high-resolution analysis of DNA methylation.

Purpose of the Study:

  • To provide an overview of emerging sequence-based DNA methylation detection techniques.
  • To categorize and describe methods based on their underlying principles.
  • To highlight the analytical steps involved in extracting methylation signatures from sequencing data.

Main Methods:

  • Overview of sequence-based assays for DNA methylation detection.
  • Categorization into affinity enrichment, chemical conversion, and enzymatic restriction methods.
  • Description of library construction, massively parallel sequencing, and data analysis pipelines.

Main Results:

  • Emerging techniques allow for comprehensive DNA methylation profiling.
  • Different methods offer varying advantages in terms of resolution, coverage, and bias.
  • Specialized analytical tools are essential for interpreting sequencing data to reveal methylation signatures.

Conclusions:

  • Sequence-based assays are revolutionizing the study of DNA methylation.
  • The choice of method depends on specific research questions and requirements.
  • Continued development in techniques and analysis will further enhance our understanding of epigenetics.