Related Experiment Video
Updated: May 14, 2026
Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
Capillary microsampling and analysis of 4-µl blood, plasma and serum samples to determine human α-synuclein
Ove Jonsson1, Ann-Charlott Steffen, Valentina Screpanti Sundquist
1Global DMPK, In Vivo Screening & Profiling, AstraZeneca R&D, SE-15185, Södertälje, Sweden. ovex.jonsson@gmail.com
Background:
The capillary microsampling technique was scaled down to enable repeated PK sampling of blood, plasma and serum from mice for the determination of the 14-kDa protein α-synuclein using the Gyrolab™ immunoassay platform.
Results:
4-µl plasma, serum or blood samples were taken from 36 mice, in total 648 samples were successfully collected and analyzed. Following intravenous administration of human α-synuclein to mice, the elimination of α-synuclein was rapid, with a half-life in plasma of 1.1 h. High endogenous levels in red blood cells in combination with some hemolysis led to a challenge in the evaluation of α-synuclein exposure in plasma and serum.
Conclusion:
The small sample volumes and flexibility in choice of liquid matrices using the capillary microsampling technique enable repeated sampling in mouse studies, as well as multi-matrix analysis if needed. Liquid microsampling is well suited for micro- and nano-liter scale immunoassays.
