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Studies on the uptake of N-methylisoquinolinium ion into rat striatal slices using high-performance liquid
1Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan.
Journal of Chromatography
|March 9, 1990
Abstract:
A simple and sensitive procedure for the measurement of N-methylisoquinolinium ion (NMIQ+), a putative neurotoxin, was devised using high-performance liquid chromatography (HPLC) with fluorescence detection. Separation of NMIQ+ was carried out by gel filtration and reversed-phase HPLC on a column of hydrophilic polymer gels (Asahipak GS-302H). The method was sensitive enough to measure 50 fmol of NMIQ+. Uptake of NMIQ+ into rat striatal slices was confirmed by this method.