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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
06:03

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Published on: September 20, 2016

Adaptive laboratory evolution for strain engineering.

James Winkler1, Luis H Reyes, Katy C Kao

  • 1Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2013
PubMed
Summary
This summary is machine-generated.

Adaptive laboratory evolution helps engineer complex traits like inhibitor tolerance in industrial organisms. This study provides detailed protocols for batch, continuous, and real-time evolution methods to aid researchers.

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Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
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Area of Science:

  • Microbiology
  • Synthetic Biology
  • Evolutionary Biology

Background:

  • Engineering complex phenotypes in industrial organisms is challenging due to limited understanding of molecular mechanisms.
  • Adaptive evolution offers a method to select for desired traits, like inhibitor resistance, by linking them to growth rate.

Purpose of the Study:

  • To provide detailed protocols for adaptive laboratory evolution to assist experimentalists.
  • To enable the engineering of complex phenotypes, such as inhibitor tolerance, in industrial model organisms.

Main Methods:

  • Detailed protocols for batch adaptive laboratory evolution.
  • Detailed protocols for continuous adaptive laboratory evolution.
  • Detailed protocols for visualizing evolution in real-time (VERT) adaptive laboratory evolution.

Main Results:

  • Established protocols for three distinct adaptive laboratory evolution approaches.
  • Facilitated the selection of mutants with enhanced tolerance to growth inhibitors.

Conclusions:

  • Adaptive laboratory evolution is a viable strategy for engineering complex phenotypes when rational design is limited.
  • The provided protocols can aid researchers in designing and executing adaptive evolution experiments for industrial microbiology and synthetic biology applications.