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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Chromosome Duplication02:05

Chromosome Duplication

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...

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Related Experiment Video

Updated: May 14, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

The RhoD to centrosomal duplication.

Athena Kyrkou1, Maria Soufi, Ramona Bahtz

  • 1Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina, Greece.

Small Gtpases
|February 21, 2013
PubMed
Summary
This summary is machine-generated.

Vesicular trafficking regulates the centrosome cycle. Our study reveals RhoD, an endosomal Rho protein, is crucial for centrosome duplication and integrity, linking it to cellular trafficking pathways.

Keywords:
Rho GTPaseRhoDcentrosomerecycling endosometrafficking

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Last Updated: May 14, 2026

Imaging Centrosomes in Fly Testes
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Published on: September 20, 2013

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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Published on: February 6, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • The centrosome, a key microtubule organizing center (MTOC), is vital for cell locomotion, sensory perception, and division in metazoans.
  • The centrosome cycle's regulation by vesicular trafficking pathways remains largely uncharacterized.
  • Emerging evidence suggests trafficking molecules influence centrosome positioning, duplication, and overall regulation.

Purpose of the Study:

  • To investigate the role of vesicular trafficking in centrosome cycle regulation.
  • To explore the function of endosomal RhoD protein in centrosome duplication.
  • To establish potential links between vesicular trafficking and centrosome structural/functional integrity.

Main Methods:

  • Functional genetic screens to identify components involved in centrosome regulation.
  • Analysis of communication networks between the nuclear envelope, Golgi, endosomal recycling compartment, and centrosomes.
  • Investigating the role of ESCRT complex proteins in centrosome function.
  • Specific examination of the endosomal Rho protein RhoD in centrosome duplication.

Main Results:

  • Functional screens identified communication between the outer nuclear envelope, Golgi, endosomal recycling compartment, and centrosomes.
  • ESCRT complex proteins are implicated in centrosome function.
  • Our study demonstrates the involvement of endosomal RhoD in centrosome duplication.
  • Evidence suggests a connection between vesicular trafficking and the centrosome's structural and functional integrity.

Conclusions:

  • Vesicular trafficking pathways are intricately linked to centrosome cycle regulation.
  • Endosomal RhoD plays a significant role in centrosome duplication.
  • Maintaining centrosome integrity and function may depend on coordinated vesicular trafficking processes.