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Updated: May 14, 2026

Spinal Cord Lateral Hemisection and Asymmetric Behavioral Assessments in Adult Rats

Spinal Cord Lateral Hemisection and Asymmetric Behavioral Assessments in Adult Rats

Published on: March 24, 2020

Sciatic nerve transection modulates oxidative parameters in spinal and supraspinal regions.

Taína Scheid¹, Lidiane Dal Bosco, Renata P Guedes

¹Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Sarmento Leite, 500, Porto Alegre, RS 90050-170, Brazil.

Neurochemical Research

|February 21, 2013

Summary

Sciatic nerve transection (SNT) in animals caused pain and functional loss. SNT altered antioxidant activity in the brain and spinal cord, revealing complex neuroaxis interactions in pain transmission.

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Modified Spared Nerve Injury Surgery Model of Neuropathic Pain in Mice

Modified Spared Nerve Injury Surgery Model of Neuropathic Pain in Mice

Published on: January 25, 2022

Area of Science:

Neuroscience
Pain Research
Oxidative Stress

Background:

Neuropathic pain is a common, debilitating condition resulting from nerve injury.
Nerve injury triggers pathological changes in pain-transmitting central nervous system regions.
Reactive oxidative species modulate nociception, and antioxidants can reduce pain.

Purpose of the Study:

To investigate changes in oxidative stress parameters in spinal and supraspinal regions after sciatic nerve transection (SNT).
To explore the relationship between antioxidant defenses in different neuroaxis regions following nerve injury.

Main Methods:

Sciatic nerve transection (SNT) model in animals.
Behavioral assessments including von Frey hairs test and sciatic functional index.
Measurement of superoxide dismutase (SOD) activity, catalase (CAT) activity, ascorbic acid (vitamin C) levels, and lipid peroxidation (LPO).

Main Results:

SNT induced mechanical allodynia and functional impairment.
Increased SOD activity observed in the cerebral cortex and brainstem 3 and 7 days post-SNT.
Elevated CAT activity in the cerebral cortex 3 days post-SNT; decreased ascorbic acid in the spinal cord 7 days post-SNT.
Increased lipid peroxidation in the cerebral cortex and brainstem 3 days post-SNT in both SNT and sham groups.

Conclusions:

Sciatic nerve transection leads to significant alterations in antioxidant enzyme activity and oxidative stress markers in both spinal and supraspinal regions.
These findings highlight the involvement of supraspinal regions in the neurobiological response to peripheral nerve injury.
The study demonstrates a complex interplay among antioxidant defense mechanisms across the neuroaxis in the context of neuropathic pain.