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Related Experiment Video

Updated: May 14, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Generating β-cells in vitro: progress towards a Holy Grail.

Benjamin H Fryer1, Alireza Rezania, Mark C Zimmerman

  • 1Janssen Research & Development, LLC, Raritan, New Jersey, USA. bfryer1@its.jnj.com

Current Opinion in Endocrinology, Diabetes, and Obesity
|February 22, 2013
PubMed
Summary

Generating functional beta-cells from stem cells offers a promising diabetes treatment. Researchers are exploring pancreatic progenitor cells and cell reprogramming to create scalable, immune-evasive therapies for improved glucose control.

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Area of Science:

  • Regenerative Medicine
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia.
  • Current treatments like pancreatic or islet transplantation face limitations due to organ scarcity.
  • Alternative strategies for generating functional beta-cells are urgently needed.

Purpose of the Study:

  • To review current advancements in generating scalable, functional beta-cells for diabetes treatment.
  • To explore the potential of human pluripotent stem cells and cell reprogramming for beta-cell replacement therapy.

Main Methods:

  • Utilizing developmental biology principles to guide human pluripotent stem cell differentiation into pancreatic progenitor cells (PPCs) in vitro.
  • Employing small molecules, growth factors, and morphogens for stepwise cell differentiation.

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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

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Last Updated: May 14, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
10:32

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

  • Investigating cell reprogramming of adult cell lineages (e.g., skin, liver) into functional beta-like cells.

    • Main Results:

    • PPCs generated in vitro can mature into functional islet-like tissues in vivo, containing diverse endocrine hormone cells.
    • Transplanted PPCs have demonstrated the ability to reverse hyperglycemia in animal models of diabetes.
    • Reprogramming of non-pancreatic adult cells into beta-like cells shows promise for alternative therapeutic avenues.

    Conclusions:

    • While fully functional beta-cells in vitro remain a challenge, PPCs and reprogrammed adult cells represent viable intermediate strategies for diabetes cell therapy.
    • Immune isolation of transplanted cells is crucial to prevent host immune rejection, with ongoing research into immune-isolation devices.
    • Future cell therapies aim to provide a scalable and potentially curative treatment for diabetes, reducing reliance on immunosuppressive drugs.