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Determination of biological peptide leukotrienes C4 and D4 by fluorometric detection combined with high-performance
Y Yamashita1, Y Uchida, A Nomura
1Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Abstract:
A fluorometric procedure combined with high-performance liquid chromatography (HPLC) has been developed to separate and quantitate peptide-leukotrienes (p-LTs) C4 and D4. The method is based on pre-column dansylation of the amino terminus of the p-LTs. Its application for the determination of the amounts of p-LTs C4 and D4 in human blood and sputum is also described. The lower limit of detection of p-LTs with this method is 1 pmol.
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