Related Experiment Video
Updated: May 13, 2026

Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule
Published on: November 25, 2015
[Incretin role in osmotic homeostasis in rats]
Abstract:
Solute-free water clearance increased by 186% in water loaded rats treated with glucagon-like peptide 1 compared to untreated group. The effect of glucagon-like peptide 1 was reproduced in experiments with its synthetic analogue exenatide. Serum osmolality in water loaded rats treated with exenatide was closer to normal level on 30, 45, 60 min of experiment compared to the untreated group and reached the control value in 60 min. Administration of glucagon-like peptide 1 receptor antagonist to water loaded rats delayed (5 ml per 100 g bw) or prevented (2 ml per 100 g bw) increase in solute-free water clearance. The obtained data suggest the involvement of glucagon-like peptide 1 in osmotic homeostasis and indicate the unique physiologic role of incretins in regulation of water balance.
Related Concept Videos
Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion
Regulation of Water Intake
Formation of Dilute Urine
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
Physiology of the Genitourinary System III: Urine Concentration and Dilution
Diabetes Insipidus II: Pathophysiology
Formation of Concentrated Urine

