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Correlation between chemosensitivity to anticancer drugs and telomerase reverse transcriptase mRNA expression in

Lin Wang1, Pei-Feng Li, Ming Geng

  • 1Department of Pathology, General Hospital of Jinan Military Command, Jinan, Shandong Province, China. gm2227@sina.com

Diagnostic Pathology
|February 26, 2013
PubMed
Abstract

Insights

Telomerase reverse transcriptase (hTERT) mRNA expression in gastric cancer (GC) correlates with drug resistance. hTERT mRNA levels can help select sensitive chemotherapy drugs for GC patients, improving adjuvant therapy outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Therapeutics

Background:

  • Determining sensitive chemotherapy drugs for gastric cancer (GC) is a major challenge in adjuvant therapy.
  • Evaluating chemosensitivity and human telomerase reverse transcriptase (hTERT) mRNA expression in GC is crucial.
  • Investigating the relationship between chemosensitivity and hTERT mRNA expression is key for personalized treatment.

Purpose of the Study:

  • To evaluate the chemosensitivity of gastric cancer (GC) cells to various anticancer drugs.
  • To examine the expression levels of human telomerase reverse transcriptase (hTERT) mRNA in GC tissues.
  • To determine the correlation between hTERT mRNA expression and chemosensitivity in GC.

Main Methods:

  • Primary culture of GC cells from 68 patients.
  • MTT assay for chemosensitivity evaluation in 60 GC cases.
  • In situ hybridization for hTERT mRNA expression in 60 GC tissues and adjacent normal mucosa.

Main Results:

  • Taxol, cisplatin, and 5-fluorouracil showed higher efficacy than adriamycin and mitomycin for GC cells.
  • Chemosensitivity was associated with tumor histological types and grade.
  • Significantly increased hTERT mRNA expression in GC tissues correlated with poorer differentiation and resistance to 5-fluorouracil or adriamycin.

Conclusions:

  • hTERT mRNA expression is significantly elevated in GC compared to normal tissues.
  • hTERT mRNA expression is linked to worse differentiation and chemoresistance in GC.
  • hTERT mRNA expression serves as a potential biomarker for selecting chemotherapeutic drugs in GC patients.

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