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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Published on: January 5, 2017
Chromatography modelling to describe protein adsorption at bead level
Spyridon Gerontas1, Michael S Shapiro, Daniel G Bracewell
1The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom.
Abstract:
Chromatographic modelling can be used to describe and further understand the behaviour of biological species during their chromatography separation on adsorption resins. Current modelling approaches assume uniform rate parameters throughout the column. Software and hardware advances now allow us to consider what can be learnt from modelling at bead level, enabling simulation of heterogeneity in bead and packed bed structure due to design or due to changes during operation. In this paper, a model has been developed to simulate at bead level protein loading in 1.5 μl microfluidic columns. This model takes into account the heterogeneity in bead sizes and the spatial variations of the characteristics of a packed bed, such as bed void fraction and dispersion, thus offering a detailed description of the flow field and mass transfer phenomena. Simulations were shown to be in good agreement with published experimental data.
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