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Updated: May 13, 2026
Development of an In Vitro Ocular Platform to Test Contact Lenses
Published on: April 6, 2016
Composition of artificial tear solution affects in vitro Pseudomonas aeruginosa biofilm formation on silicone
Mukesh Kumar Yadav1, Roy S Chuck, Choul Yong Park
1Department of Ophthalmology, Ilsan Hospital, Dongguk University, Koyang, Kyunggido, South Korea.
Purpose:
To investigate Pseudomonas aeruginosa biofilm formation on silicone hydrogel contact lens in various artificial tears.
Methods:
P. aeruginosa was cocultured with contact lenses (Senofilcon A, Acuvue Oasys Hydraclear plus(®); Johnson & Johnson, Jacksonville, FL) in artificial tears containing hyaluronic acid (HA) (0.3%, 0.1%, or 0.1%+benzalkonium chloride) or carboxymethylcellulose (0.5% or 0.3%+dextran). P. aeruginosa biofilm was stained with crystal violet (0.1%) after 48 h of incubation. The optical density of the dissolved biofilm was measured by a spectrophotometer at 570 nm and was compared.
Results:
P. aeruginosa biofilm formation in 3 solutions (0.3% HA, 0.1% HA+benzalkonium chloride and 0.3% carboxymethylcellulose+dextran) was significantly decreased compared to the other 2 solutions (0.1% HA and 0.5% carboxymethylcellulose) (P<0.01).
Conclusions:
P. aeruginosa biofilm formation on silicone hydrogel contact lens was affected by the composition of artificial tears. A higher concentration of HA and addition of benzalkonium chloride or dextran in artificial tears were helpful to prevent P. aeruginosa biofilm formation.
