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Updated: May 13, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
Free amino acid profiling in grain amaranth using LC-MS/MS
Mansingraj S Nimbalkar1, Sandeep R Pai, Nilesh V Pawar
1Laboratory of Cytogenetics and Plant Breeding, Department of Botany, Shivaji University, Kolhapur 416 004, Maharashtra, India. mansingrajsu@gmail.com
This study analyzed free amino acids in amaranth grain using LC-MS/MS, finding higher essential amino acids like Threonine and Tryptophan. This method bypasses traditional derivatization for accurate grain amaranth amino acid profiling.
Area of Science:
- Agricultural Science
- Biochemistry
- Food Science
Background:
- Accurate amino acid analysis often relies on derivatization.
- Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) offers potential for direct analysis.
- Free amino acid content in grain amaranth is not well-documented, hindering understanding of its physiological roles.
Purpose of the Study:
- To quantify the free amino acid composition of grain amaranth.
- To evaluate the utility of LC-MS/MS for analyzing amino acids in amaranth grain without derivatization.
- To provide baseline data on essential and non-essential amino acids in amaranth.
Main Methods:
- Utilized Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) for amino acid analysis.
- Analyzed free amino acid pool directly within the amaranth grain.
- No chemical derivatization step was employed prior to analysis.
Main Results:
- Identified and quantified various free amino acids in grain amaranth.
- Observed a higher concentration of essential amino acids, particularly Threonine and Tryptophan, compared to non-essential amino acids.
- Demonstrated the feasibility and accuracy of using LC-MS/MS for direct amino acid profiling in amaranth grain.
Conclusions:
- LC-MS/MS is a viable and accurate method for analyzing free amino acids in amaranth grain, potentially replacing derivatization techniques.
- Grain amaranth exhibits a notable profile rich in essential amino acids, highlighting its nutritional value.
- This study provides the first report on amaranth grain's free amino acid composition using LC-MS/MS.
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