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Kinetics of a multistate enzyme in a large oscillating field
1Chemical Process Metrology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.
Biophysical Journal
|April 1, 1990
Abstract:
A simple, general, and efficient method for calculating the response of a set of coupled first-order (or pseudo-first-order) chemical reactions to an arbitrarily large periodic field is described. The method is applied to a four-state membrane transport enzyme that is electroconformationally coupled to an ac field, i.e., the enzyme has electric charges that move concomitantly with a conformational transition. The calculation is done both for enzymes in a planar membrane and for enzymes in the spherical membrane of a cell or vesicle in suspension.