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Related Concept Videos

Adsorption Isotherms I01:29

Adsorption Isotherms I

Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed molecules.Consider the...
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Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...

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Quantifying nisin adsorption behavior at pendant PEO layers.

Justen K Dill1, Julie A Auxier, Karl F Schilke

  • 1School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331-2702, United States.

Journal of Colloid and Interface Science
|March 1, 2013
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Antimicrobial peptide nisin effectively coats medical devices, showing potent activity against Gram-positive bacteria. Nisin adsorption and release depend on polyethylene oxide (PEO) brush layer characteristics, influencing antibacterial strategies.

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Antimicrobial Peptides

Background:

  • Nisin, an antimicrobial peptide, exhibits strong activity against Gram-positive bacteria, including common implant-associated pathogens.
  • Its mechanism of action reduces resistance development, and it shows low human toxicity, indicating potential for medical device coatings.
  • Understanding nisin loading and release from polyethylene oxide (PEO) brush layers is crucial for optimizing drug delivery systems.

Purpose of the Study:

  • To quantitatively assess nisin loading and release from PEO brush layers using optical waveguide lightmode spectroscopy.
  • To investigate the influence of PEO chain length on nisin adsorption, desorption, and clustering behavior.
  • To evaluate nisin's interaction with both uncoated and PEO-coated silica surfaces.

Main Methods:

  • PEO brush layers were created by radiolytic grafting of Pluronic® F108 or F68 onto silanized silica surfaces.
  • Optical waveguide lightmode spectroscopy (OWLS) was employed to monitor mass changes during nisin adsorption-elution cycles.
  • Kinetic data were analyzed using a model for history-dependent adsorption to determine rate constants and clustering effects.

Main Results:

  • Nisin adsorbed to uncoated and F108-coated surfaces but not to F68-coated surfaces.
  • Adsorbed nisin exhibited greater resistance to elution from uncoated surfaces.
  • Lateral rearrangement and clustering of nisin were observed only on the uncoated surface.

Conclusions:

  • Nisin entrapment on F108-coated surfaces is influenced by a hydrophobic inner PEO region.
  • Shorter PEO chains (F68) were insufficient for effective nisin entrapment.
  • Surface characteristics, particularly PEO brush layer properties, significantly impact nisin's adsorption, elution, and clustering behavior.