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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: May 13, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

A CRISPR way to engineer the human genome.

Sivaprakash Ramalingam, Narayana Annaluru, Srinivasan Chandrasegaran

    Genome Biology
    |March 2, 2013
    PubMed
    Summary

    The CRISPR/Cas system enables precise genome engineering in mammals. This RNA-guided technology offers a versatile tool for targeted DNA modification in various mammalian cells.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • The type II prokaryotic CRISPR/Cas system is a powerful tool for genome editing.
    • Targeted manipulation of mammalian genomes is crucial for research and therapeutic applications.

    Purpose of the Study:

    • To demonstrate the efficiency and versatility of RNA-guided genome engineering in mammalian systems.
    • To highlight the potential of the CRISPR/Cas system for targeted DNA modification.

    Main Methods:

    • Utilizing the type II CRISPR/Cas system with specific RNA guides.
    • Applying the system for targeted manipulation of mammalian genomes.

    Main Results:

    • Achieved efficient and versatile genome engineering in mammalian cells.

    More Related Videos

    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

    Published on: May 30, 2025

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
    09:51

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

    Published on: May 25, 2018

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    Last Updated: May 13, 2026

    Genome Editing in Mammalian Cell Lines using CRISPR-Cas
    07:56

    Genome Editing in Mammalian Cell Lines using CRISPR-Cas

    Published on: April 11, 2019

    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
    07:49

    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

    Published on: May 30, 2025

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
    09:51

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

    Published on: May 25, 2018

  • Demonstrated successful targeted DNA modification using the CRISPR/Cas system.

    • Conclusions:

    • The CRISPR/Cas system is an effective method for RNA-guided genome engineering in mammals.
    • This technology offers a versatile approach for targeted manipulation of mammalian genomes.