Related Experiment Video
Updated: May 13, 2026

Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
Published on: January 24, 2025
Complete genome sequence of Treponema pallidum strain DAL-1
Marie Zobaníková1, Pavol Mikolka, Darina Cejková
1Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Abstract:
Treponema pallidum strain DAL-1 is a human uncultivable pathogen causing the sexually transmitted disease syphilis. Strain DAL-1 was isolated from the amniotic fluid of a pregnant woman in the secondary stage of syphilis. Here we describe the 1,139,971 bp long genome of T. pallidum strain DAL-1 which was sequenced using two independent sequencing methods (454 pyrosequencing and Illumina). In rabbits, strain DAL-1 replicated better than the T. pallidum strain Nichols. The comparison of the complete DAL-1 genome sequence with the Nichols sequence revealed a list of genetic differences that are potentially responsible for the increased rabbit virulence of the DAL-1 strain.
Related Concept Videos
Sanger Sequencing
Bacterial Phylum Spirochaetes

