Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Determination of hemoglobin-oxygen affinity on micro samples.

J Lyonnais, D E Lott, J W Kane

    The Journal of Laboratory and Clinical Medicine
    |February 1, 1975
    PubMed
    Summary

    This study introduces a refined spectrophotometric method for measuring hemoglobin-oxygen dissociation using minimal whole blood. Bovine serum albumin in bicarbonate buffer significantly impacts the P50 value, suggesting an influence on intracellular pH.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Should Free Thyroxine Go Back into the Routine Thyroid Profile?

    Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2015
    Same author

    Suspected collagen disorders in the bleeding disorder clinic: a case-control study.

    Haemophilia : the official journal of the World Federation of Hemophilia·2012
    Same author

    Acute effects of varying doses of propranolol upon oxygen haemoglobin affinity in man.

    British journal of clinical pharmacology·2012
    Same author

    Generational differences in factors influencing physicians to choose a work location.

    Rural and remote health·2012
    Same author

    First successful unrelated bone marrow transplant in Canada: A look at the longest surviving recipient.

    Canadian family physician Medecin de famille canadien·2011
    Same author

    Letter from Ontario: Research at long odds.

    British medical journal·2010

    Area of Science:

    • Hematology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate measurement of hemoglobin-oxygen dissociation is crucial for understanding oxygen transport.
    • Existing methods for determining the hemoglobin-oxygen dissociation curve can be resource-intensive.

    Purpose of the Study:

    • To modify and validate a spectrophotometric method for determining the hemoglobin-oxygen dissociation curve.
    • To investigate the effect of bovine serum albumin on the P50 value in different buffer systems.

    Main Methods:

    • Spectrophotometric determination of hemoglobin-oxygen dissociation on dilute red cell suspensions.
    • Equilibration of red cell suspensions with controlled air/nitrogen and CO2 gas mixtures.
    • Measurement of P50 values in various buffer conditions, including the presence of albumin and polyvinyl-pyrrolidone (PVP).

    Related Experiment Videos

    Main Results:

    • The modified method accurately measures the hemoglobin-oxygen dissociation curve using 10-20 µL of whole blood.
    • The P50 value was determined to be 25.9 ± 1.5 S.D., comparable to established methods.
    • Addition of bovine albumin (≥35 mg%) increased the P50 by 3 mm Hg, particularly in bicarbonate buffer.

    Conclusions:

    • The refined spectrophotometric method offers a sensitive and efficient approach for hemoglobin-oxygen dissociation analysis.
    • Bovine serum albumin significantly influences the P50 value, suggesting a potential role in modulating intracellular pH.
    • The findings highlight the importance of buffer composition and additives in accurate P50 determination.