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[Identification of herba rabdosiae serrae from different plant resources by RAPD method]
Xiao-lu Mo1, Qing-qian Zeng, Shan-shan Huang
1Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China.
Objective:
To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis.
Methods:
The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses.
Results:
12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map.
Conclusion:
RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.
