Related Experiment Video
Updated: May 13, 2026
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
[Study on the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase
Yu-jun Sun1, Zheng-peng Li, Zhong-you Ma
1School of Life Science, Anhui Science and Technology University, Fengyang 233100, China. sunyujun320@yahoo.com.cn
Objective:
To study the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase for increasing of yield.
Methods:
On the basis of single factor tests, orthogonal experiment design was applied to analyze the influence of factors including enzyme dosage, enzymolysis time, enzymolysis temperature and material/liquid ratio on the extraction rate of the polysaccharides.
Results:
The optimal extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta was as follows: enzyme dosage was 1.5%, enzymolysis time was 2 h, enzymolysis temperature was 40 degrees C, and material/liquid ratio was 1:15. The extraction rate of polysaccharide was 11.26% under the extraction conditions.
Conclusion:
The process is simple, stable and practicable, and can be used for the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta.
