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Related Experiment Video

Updated: May 13, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

[An enzyme reactor based on aptamer modified microfluidic chip for protein analysis].

Peng Xiao1, Dalei Li, Yan Man

  • 1School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

Se Pu = Chinese Journal of Chromatography
|March 5, 2013
PubMed
Summary

This study introduces a novel aptamer-immobilized enzymatic chip for rapid protein analysis. The developed online platform demonstrates high sensitivity and stability for efficient proteomics research.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Context:

  • Aptamers are versatile recognition molecules with broad scientific applications.
  • Immobilized enzymatic reactors offer high digestion efficiency and seamless integration with separation/detection systems.

Purpose:

  • To develop a novel microfluidic enzymatic chip immobilizing trypsin using aptamers.
  • To establish an online analysis platform combining the aptamer-based chip with high-performance liquid chromatography-tandem mass spectrometry for protein analysis.

Summary:

  • A microfluidic chip immobilized trypsin via aptamers, integrated into an online platform with LC-MS/MS.
  • The system achieved rapid sample digestion (5.76 s) and successfully identified low protein amounts (5 ng myoglobin with 37% sequence coverage).

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Last Updated: May 13, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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  • High sequence coverages (up to 65.0%) and low relative standard deviations (as low as 2.7%) were observed for mixed protein analysis.

    • Impact:

    • The online platform exhibits high sensitivity and stability, offering a promising tool for rapid and high-throughput proteomics.
    • This technology facilitates efficient protein analysis and characterization in complex biological samples.
    • The aptamer-based immobilization strategy enhances enzymatic reactor performance for advanced proteomic studies.